Strengthens enforcement of the Animal Welfare Act by expanding prohibitions on selling or transporting animals without a valid license, clarifying inspection and seizure authority, and giving the Attorney General explicit civil enforcement powers in federal court (including civil actions, fines, and seizures). It requires the Department of Agriculture and the Department of Justice to enter a memorandum of understanding within 180 days to coordinate enforcement and allows penalty funds to be used to pay reasonable temporary care costs for animals pending legal proceedings. The bill also adds a severability rule and affirms that federal courts and the Attorney General have jurisdiction and authority to enforce the Act and regulations made under it.